rabbit polyclonal anti eif4a3  (Bioss)

Journal: iScience

Article Title: circIFNGR2 regulating ankylosing spondylitis-associated inflammation through macrophage polarization

doi: 10.1016/j.isci.2023.107325

Figure Lengend Snippet: eIF4A3 regulated the expression of circIFNGR2 (A) The binding sites of eIF4A3 in the flanking sequences of the IFNGR2 pre-mRNA transcript were predicted by circInteractome. (B) RIP assays for detection of the binding relationships among eIF4A3 and IFNGR2 pre-mRNA transcripts. (C) Western Blot analysis for identification of the binding relationships among eIF4A3 and the flanking sequences of IFNGR2 pre-mRNA transcripts. (D) qRT-PCR analysis of the expression level of eIF4A3 mRNA in THP-1 cells, M0 macrophages, M1 macrophages, and M2 macrophages. (E) qRT-PCR analysis of the expression level of eIF4A3 mRNA, circIFNGR2, and linear IFNGR2 with or without overexpression and knockdown of eIF4A3. (F) qRT-PCR analysis of the expression of circIFNGR2 in PBMCs from 24 healthy controls and 33 patients with AS. (G) Correlation analysis of the expression levels of circIFNGR2 and eIF4A3 mRNA in PBMCs from patients with AS. Data in B, D, and E are presented as mean ± SD, data in F are presented as median and quartiles. ∗∗: p < 0.01; ∗∗∗: p ＜0.001; ns: p ≥ 0.05.

Article Snippet: Rabbit polyclonal anti-eIF4A3 , Bioss , Cat# bs-14548R.

Techniques: Expressing, Binding Assay, Western Blot, Quantitative RT-PCR, Over Expression, Knockdown

Journal: iScience

Article Title: circIFNGR2 regulating ankylosing spondylitis-associated inflammation through macrophage polarization

doi: 10.1016/j.isci.2023.107325

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-eIF4A3 , Bioss , Cat# bs-14548R.

Techniques: Virus, Recombinant, Adjuvant, In Vivo, SYBR Green Assay, Transfection, Lysis, Blocking Assay, Western Blot, DNA Extraction, Protein Extraction, Enzyme-linked Immunosorbent Assay, Purification, RNA Immunoprecipitation, Luciferase, Software

Journal: Cell reports

Article Title: The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton

doi: 10.1016/j.celrep.2024.114666

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-eIF4AIII/EIF4A3 , Bethyl , Cat#A302-980A; RRID:AB_10749033.

Techniques: Virus, Recombinant, Electron Microscopy, Protease Inhibitor, Binding Assay, Spin Down Assay, Control, Plasmid Preparation, Software

Journal: Cell reports

Article Title: The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton

doi: 10.1016/j.celrep.2024.114666

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-eIF4AIII/EIF4A3 , Santa Cruz Biotechnology , Cat#sc-67369; RRID:AB_2097516.

Techniques: Virus, Recombinant, Electron Microscopy, Protease Inhibitor, Binding Assay, Spin Down Assay, Control, Plasmid Preparation, Software

Journal: Cell reports

Article Title: A bidirectional competitive interaction between circHomer1 and Homer1b within the orbitofrontal cortex regulates reversal learning.

doi: 10.1016/j.celrep.2021.110282

Figure Lengend Snippet: Figure 6. EIF4A3 binds to circHomer1 and upregulates its expression (A) Relative to control EIF4A3 mRNA levels from the OFC of BD patients. *p < 0.05, based on a univariate general linear model corrected for RIN, PMI, brain pH, and age. (B) Correlations between relative to control changes in circHomer1 in the OFC of patients with SCZ and BD (SMRI cohort, RNaseR-treated samples used for circHomer1 detection) and the relative to control ratios of EIF4A3 mRNA levels. Spearman correlation coefficient and two-tailed p values are shown in the graph. (C) Relative to D50 Eif4a3 mRNA levels in non-injected adult mouse OFC during different stages of the touch-screen reversal learning paradigm. **p < 0.01, two- tailed, one-sample t test relative to D50 levels. (D and E) RIP using an antibody against EIFA3 protein. Western blotting data are shown in (D). Mean ± SEM relative to immunoglobulin G (IgG) control circHomer1 and circCrebbp levels (based on quantitative real-time PCR and normalized to 18S rRNA) after EIF4A3 RIP (E). *p < 0.05, two-tailed, one-sample t test relative to IgG control levels. (F and G) Relative to vehicle circHomer1 and circTulp4 levels 24 h after treatment with EIF4A3 inhibitor in mouse primary cortical neurons (F) and human HEK293T cells (G). *p < 0.05 and **p < 0.01, two-tailed, one-sample t test relative to vehicle. (H and I) Relative to vehicle circHomer1, circCDR1as, and EIF4A3 mRNA levels 48 h after shRNA-mediated KD of EIF4A3 in human HEK293T cells (H) and human differentiated SH-SY5Y cells (I). *p < 0.05 and ****p < 0.0001, two-tailed, one-sample t test relative to vehicle. All data are shown as mean ± SEM based on quantitative real-time PCR and normalization to 18S rRNA. The individual biological replicates are shown within each graph.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit polyclonal anti-eIF4A3 Bethyl Laboratories A302-981A Bacterial and virus strains pLV-mU6-SYN-GFP high titer lentiviruses Systems Biosciences N/A Biological samples Human postmortem brain total RNA samples from the OFC and DLPFC of subjects with BD N=30), SCZ (n=32), or unaffected Control subjects (n=33) SMRI, Torrey et al. (2000) N/A An additional smaller cohort of postmortem brain total RNA samples from OFC of subjects with BD (n=21), SCZ (n=20) or unaffected Control subjects (n=20) Harvard Brain Tissue Resource Center (HBTRC) N/A A larger postmortem brain total RNA cohort (DLPFC) (N=54 for SCZ, N=40 for BD, and N=60 for Controls) Icahn School of Medicine at Mount Sinai N/A Crude synaptosomes were extracted from the OFC of mice Boese et al. (2016); Rao and Steward (1991) N/A iPSC-derived neuronal cultures extracted from 4 BD patients and 3 unaffected Controls Center for Genomic Medicine at the Department of Psychiatry ofMassachusetts General Hospital (Zimmerman et al., 2020) N/A iPSC derived neuronal cultures derived from 9 childhood-onset SCZ and 10 healthy Controls Mount Sinai Icahn School of Medicine (Hoffman et al., 2017; Zimmerman et al., 2020) N/A B lymphoblastoids (BLCL) were collected from blood of BD patients and healthy Controls (Squassina et al., 2013) N/A Chemicals, peptides, and recombinant proteins eIF4A3-IN-2 (EIF4A3 inhibitor) MedChemExpress Cat#: HY-101785 DMEM, MEM, Fetal Bovine Serum, Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture ThermoFisher Scientific Cat#: 12430112, 11095080, A4766801, 15640055 Retinoic acid RA Sigma-Aldrich R2625 NeurobasalTM Plus Medium, B-27TM Supplement (50X) ThermoFisher Scientific Cat#: A3582901, 17504044 LipofectamineTM 3000 / P3000 reagent ThermoFisher Scientific Cat#: 15338030 RNAseOUT Ribonuclease Inhibitor Invitrogen Cat#: 10777019 Lithium Chloride Solution, 1M Fisher Science EducationTM Cat#: S25390 Critical commercial assays miRNeasy RNA isolation kit Qiagen, Hilden, Germany Cat#: 217004 SuperScript IV First-Strand Synthesis System Thermo Fisher Scientific Cat#: 18091200 PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat#: A25471 TaqMan Gene Expression Assays Thermo Fisher Scientific Cat#: 4331182 Pierce Magnetic streptavidin beads Thermo Fisher Scientific Cat#: 88816 NucleoSpin RNA Mini kit Machery-Nagel REF 740984.50 Kapa Stranded RNA-seq Library kit Illumina, San Diego, CA N/A (Continued on next page) e1 Cell Reports 38, 110282, January 18, 2022

Techniques: Expressing, Control, Two Tailed Test, Injection, Western Blot, Real-time Polymerase Chain Reaction, shRNA

Journal: Cell reports

Article Title: A bidirectional competitive interaction between circHomer1 and Homer1b within the orbitofrontal cortex regulates reversal learning

doi: 10.1016/j.celrep.2021.110282

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: rabbit polyclonal anti-eIF4A3 , Bethyl Laboratories , A302-981A.

Techniques: Virus, Control, Derivative Assay, Recombinant, Isolation, SYBR Green Assay, Gene Expression, Reverse Transcription, Sequencing, shRNA, Plasmid Preparation, Software